Monday, January 29
FYP project's been confirmed.
I won't be having any holidays.
:(
Project title : Optimisation of High Throughput In vitro Antiinflammatory
Assay
(A) Project Objectives:
1. To develop and optimize a high throughput in vitro anti-inflammatory
assay (lipoxygenase inhibition activities)
2. To apply the optimized assay in determining inflammatory activities of
plants/food compounds
3. To isolate and detect the active protein metabolites found in plants/food
(B) Project Description:
(Please indicate if project involves human volunteers; IRB application is required.) This project aims to develop and optimize a high throughput colorimetric in vitro anti-inflammatory assay. The colorimetric lipoxygenase assay is based on FOX assay (Ferric Oxidation of Xylenol Orange). The assay is based on the principle that under acidic conditions, a lipid hydroperoxide can oxidize Fe2+ to Fe3+ ions which then oxidizes xylenol orange to form a product that absorbs strongly at 560 nm. Thus, the increased absorbance readings observed indicates an increase amount of the LOX-catalysed products in the mixture. The absorbance readings for control well and test sample well will be collected and used to calculate the percentage inhibition of test sample.
Choice of substrate concentration, enzyme concentration and FOX reagent composition will be investigated to generate the highest possible signal-to-noise ratio. The sensitivity of the assay will be assessed using hydrogen peroxide and will be further validated using known lipoxygenase inhibitors. The optimized assay will then be applied in screening for selected potential active plants and food such as curcumin, ginger, garlic and soybean.
The protein of the plant/food samples will then be extracted and characterized using the SDS-PAGE and/or Thin Layer Chromatography technique. Observation will be made for common protein compounds that exhibit anti-inflammatory properties.
Whaaaaaaaaaaaaaaaaaaat?
Funeral For A Friend - History
Dear friends of this academy, ohh
Romeo is bleeding to death
To see a friend bleed to death what for
Some kind of metaphor that I can't see?
So, I'll drink until I see it
This sky will make me sick
So, I'll give up on you
I'll give up on this
This sky will make me sick
I'll give up on this
I'll give up on you
Archers in your arches
Raise your fingers for one last salute
And bleed this skyline dry
Your history is mine
So you want to hold me up and bring me down
Yes, you want to hold me up and break me down
I don't care for your sweet scent or,
The way you want me more than I want you
I don't care for your sweet scent or,
The way you want me more than I want you
Archers in your arches
Raise your fingers for one last salute
And bleed this skyline dry
Your history is mine
Archers in your arches
Raise your fingers for one last salute
And bleed this skyline dry
Your history is mine
It's all mine
It's all mine
It's all mine
Archers in your arches
Raise your fingers for one last salute
And bleed this skyline dry
Your history is mine
Archers in your arches
Raise your fingers for one last salute
And bleed this skyline dry
Your history is mine
Saturday, January 20
Friday Night/Saturday Morning
Met Sze at 9pm and had my dinner at Suntec's Macs. Lena and Ant came promptly at 10pm and we headed over to DXO.
On the way, we saw Prissy-Khoo who irritated me so much on Sze's 18th that I wanted to scratch her with my keys.
The DXO bouncers made me pay the $20 entry fee(it was ladies's night) and I wasn't entitled to free drinks cause I "dressed like a man" and it wasn't "fair to the girls inside". Wth?
We saw Apphia, Audrey Chen and Ying Jie at DXO.
Had 2 tequila limes ($20 entry fee + 2 free drinks)
Danced till we dropped (lol!)
Had makan at the food centre next to DXO around 2am
Had half a can of Kilkenny.. It wasn't as nice as I remembered it. Maybe its cause I didn't follow the "pour contents into a glass immedieately after opening".
Felt a little dizzy after.
We were waiting for Sze to grab a cab outside DXO cause she wanted to NETS her cabfare but the uncle said cannot so she got out and we stoned for a second.
Suddenly, a red car went speeding by, lost control, drifted and crashed into a street sign causing it to collaspe.
I took out my phone and dialled 995 but when I saw the car reversing as if to drive off, I hung up.
However, the driver just proceeded to crash into the bushes on the other side of the road.
This time, I just dialled 995 and told the dispatch where we were and what happened.
A bunch of guys came sprinting out of DXO over to the wrecked car. There was a lot of smoke coming out from under its hood but the guys got it under control.
The four of us just slumped at the roadside trying to shake off the shock that was caused by witnessing the accident.
Finally, after about 5 minutes, two traffic police troopers came, followed after awhile by an ambulance.
After witnessing the driver being brought out from his car to the ambulance in a stretcher, we headed home. He didn't seem in bad shape.
Sze hopped on a cab and Lena, Ant and I took the night rider(SMRT).. But it broke down near MOS/Liang Court. Lena was so freaked out on the bus, saying that it was like Final Destination 4 or something.. lol
We visited Liang Court's toilet cause I needed to pee only to find it infested with baby cockroaches. Then we tried Liang Court's disabled toilet but had no working lights.
I went to pee anyway and we hopped on the next night rider(SBS) and had to pay a new fee($3) cause the driver said that our bus was SMRT.
What crap's that? $6 for a busride home!
Got home around 4.45am.
Thank gosh mum didn't ask much about that night.
I've been obsessed with the movie Step Up over the weekend.
In my opinion, the dance club scene's the best one of the movie.
Channing Tatum and Jenna Dewan are hot when the dance man!
Drew Sidora - Til The Dawn
Oh Oh
Oh Oh
Oh Oh
Oh Oh
Ohhhhhhh
Oh no no no
Out on the weekend
Out on the weekend
Out on the weekend
Out on the weekend
Back again, hit the lights, let party begin
Mix a little love, get the mood jumpin
From New York to Miami by ten
I think I'm ready to go, hot...
High, every time that we hit up our spot
See everyone in line, and they're payin a lot
Drop a Ben, them drinks are rich
But the music is free
You want it, you love it
You need it, but you know you gotta have it boy
The music is playin'
(Out on the weekend, Out on the weekend, weekend)
This is for the girls comin' from NYC
I'm feelin' you like yous family
And everybody down from LA to DC
Rockin' to the same old-school beat
This is for the kids in the clubs and the street
Turn teh volume up 3db
Get on the dance floor where the music is free
Slip, on until the dawn's gone with me
Gone until the dawn's gone with me
Where the music is free
Slip, on until the dawn's gone with me
(Out on the weekend, on the weekend, weekend)
[spoken:]
Now all my ladies,
Move it to the front,
show 'em what you got
Ooh, ooh, ooh
Oh, Oh ooh
Oh, Oh ooh
Oh, Oh ooh, ooh, ooh
Fellas?
Move, like that
Oh, Oh (x4)
[Mario:]
I like that
And all my ladies say (Ah, Ah)
And all the fellas say (Ah, Ah)
Everybody say (Ah, Ah)
Come dance with me, ohh (Ah Ah)
[Drew:]
And the story goes just like this
We pack 'em till the morning, there'll be no lettin' off
Dollars droppin onto the table tops
You can shake your thing and there aint no cost
This is for the girls comin' from NYC
I'm feelin' you like yous family
And everybody down from LA to DC
Rockin' to the same old-school beat
This is for the kids in the clubs and the street
Turn teh volume up 3db
Get on the dance floor where the music is free
This is for the girls comin' from NYC
I'm feelin' you like yous family
And everybody down from LA to DC
Rockin' to the same old-school beat
This is for the kids in the clubs and the street
Turn teh volume up 3db
Get on the dance floor where the music is free
Slip, on until the dawn's gone with me
Gone until the dawn's gone with me
Where the music is free
Slip, on until the dawn's gone with me
(Out on the weekend, on the weekend, weekend)
Friday, January 19
For the five lives lost today because of me.
I'm so sorry.
Jimmy Eat World - Hear You Me
There's no one in town I know
You gave us some place to go
I never said thank you for that
I thought I might get one more chance
What would you think of me now,
So lucky, so strong, so proud?
Never said thank you for that
Now I'll never have a chance
May angels lead you in
Hear you me my friends
On sleepless roads, the sleepless go
May angels lead you in
So what would you think of me now
So lucky, so strong, so proud?
I never said thank you for that
Now I'll never have a chance
May angels lead you in
Hear you me my friends
On sleepless roads, the sleepless go
May angels lead you in
May angels lead you in
May angels lead you in
May angels lead you in
May angels lead you in
And if you were with me tonight
I'd sing to you just one last time
A song for a heart so big
God couldn't let it live
May angels lead you in
Hear you me my friends
On sleepless roads, the sleepless go
May angels lead you in
May angels lead you in
Hear you me my friends
On sleepless roads, the sleepless go
May angels lead you in
On sleepless roads, the sleepless go
May angels lead you in
Now I'll never have a chance to say
I'm sorry.
Thursday, January 18
I feel like shit.
I want to quit.
Fuck people who abandon their pets.
Sunday, January 14
Hi,
I'm just back from yet another wake.
Its scary to see people going without warning.
=/
Take care buddy.
Suffering from reeeeeally sore shoulders.
Mapling less and less.
Gone back to novel reading.
Busy with HRSS stuff.
Trying my best in school.
Drinking less Coke.
Sleeping more.
Spending less money.
Missing someone.
To all my friends,
You've each played a different part in shaping my character and helping me to be who I am now. I'm grateful to have known each one of you no matter how limited our time together was. Even if we don't talk now, it doesn't mean that I've forgotten you or taken you for granted. Sometimes I may say or do things I don't mean. Please accept my apologies if I have done anything to hurt you. Every single thing that has happened to me has changed me, regardless of good or bad, I guess I'm glad that they happened. (saying all this now because I know I may never get a chance to tell you guys this in future:) You each mean something to me. Love you all. (:
Friday, January 12
The Ataris - Summer Wind Was Always Our Song
These break-up songs make sense again
And I really wish they didn't.
Sinatra's singing summer wind
And I'm thinking of the night we met.
Just one last time
Can I hear you say?
You're my little boy
I never want you to go away
Where are you?
Please believe in me.
I'm not hanging up the phone
'til I hear you say,
I love you.
I need you near.
Just give me one last chance
And I'll never let you down again.
Oh and what I wouldn't give
Just to kiss your lips again
To hold your hand next to my heart
And wake up with you in our apartment.
Just one last time
Can I call you my sweetheart
My best friend
Why do all good things come to an end.
Where are you?
Please believe in me.
I'm not hanging up the phone
'til I hear you say,
I love you.
I need you near.
Just give me one last chance
And I'll never let you down again.
Friday, January 5
Your silence kills me.
Help me to forget.
Decide to be either naughty or nice.
Don't stay in between.
God, I miss you.
Close your eyes, make a wish
This could last forever
If only you could stay, with me now
So tell me what it is that keeps us from each other now
Yeah, its coming to get me
You're under my skin
No I can't let you go
You're a part of me now
Caught by the taste of your kiss
And I don't wanna know the reason why I can't stay forever like this
Now I'm climbing the walls cuz I miss, you
Thursday, January 4
(2.44am)
School in about 6 hours!
Its only five weeks its only five weeks its only five weeks.
Tuesday, January 2
Its only the start of the second day of 2007 and already I'm feeling like an emo shitfuck.
I'm tired, hungry and so so alone.
We were both 18 and it felt so right
Sleeping all day, staying up all night
Staying up all night
If I could find you now things would get better
We could leave this town and run forever
Let your waves crash down on me and take me away